Does anyone know a commercial source of adenoviral expression vector to express the interferon-alpha?

More Divaker Choubey's questions See All

What is known about the role of p53 pathway in the development of colorectal cancer (CRC) using mouse models?

In chemical-induced colitis-associated cancer mouse models, treatment of mice with azoxymethane (AOM; a mutagen and carcinogenic compound) and dextran sodium sulphate (DSS) in colon epithelial...

03 April 2016 5,477 4 View

What do we know about the role of inflammasomes in the development of inflammation-associated human cancers?

Studies in certain mouse models have indicated that activation of inflammasomes protects against the development of cancers. However, the role of inflammasomes remains unclear in the development...

01 February 2015 9,701 7 View

What are most acceptable mouse models of Alzheimer's disease?

We would like to use a mouse model(s) to explore the potential role of certain signaling pathways that are known to contribute to inflammation.

09 October 2014 9,689 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

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Prem Subramaniam

Here you go : from Vigene Biosciences. This has a FLAG and His-Tag.

http://www.vigenebio.com/search/view.php?aid=22679

Divaker Choubey

Thank you Prem for your help.

Anne von Koschembahr

What about ordering vectors from the CCHMC core? You are already at the university and their vectors are usually much cheaper than ordering commercial products. No shipping cost since they are made local.

https://research.cchmc.org/translationalcores/vector-production/gmp-vector-production