Does anyone have treated A549 cell with IL-13 to induce periostin release?

12 February 2015 1 8K Report

In many papers it is mentioned that IL-13 can induce periostin release from airway epithelial cells. But I did not find about the A549 cell. Is there any information regarding the release of periostin from A549 cell after IL-13 treatment?

Oscar L. Sierra

The best approach is to have at least two types of cell cultures: 1-regular submerged cells attached to plastic or collagen coated multiwell plates and treat with a known dose of IL-13 and harvest cells at different times for RNA and Western blot. 2-Differentiate cells in air-liquid interfase cultures and do a likewise treatment. If IL-13 does not stimulate periostin mRNA synthesis or protein accumulation then it's unlikely that you'll figure out the proper conditions.

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