Aoa Zahid,

If you are unable to find any antibody, I would suggest you to align the sequences of chicken vs other species and then have a look on the sequence similarity and find out the antibody that least cross reacts. This might give you an idea to test that one on your samples.

These might be helpful to you.

https://www.jstage.jst.go.jp/article/jvms/66/7/66_7_807/_pdf, http://www.ncbi.nlm.nih.gov/pubmed/24863561, http://www.sciencedirect.com/science/article/pii/S1045105607000243

Good luck

Khalid

Walaikum us salam and thanks ever so much Khalid brother...the problem i have in china is that i cant really open NIH site or any other site where i can match chicken with closely related species...anyhow thanks again...

So far nobody has been able to show that chicken can actually get a prion disease. Therefore, there should be no PrPSc in chicken brain.

Of course, if you would be the first to convincingly show that your chickens actually developed a prion disease, that would be an important and high impact paper.

so it simply means if i give any prpsc to chicken it will not develop disease?

Holger is right, so far no body is able to show prion disease in chicken (http://www.ncbi.nlm.nih.gov/pubmed/22093239). But it doesn't exclude the possibility in future.

Sure if you can show a prionopathy in chicken, you may get a good impact paper like the one with prion disease in rabbits by J. Castilla group http://www.ncbi.nlm.nih.gov/pubmed/22416127, but this was done by using the protein misfolding cyclic amplification. Rabbit were considered for long time resistant to prion infection. PMCA is a PCR of proteins.

thanks ever so much mr holger and mr salamat

Hi,

We have a very good polyclonal antibody that recognizes extremely well the chicken PrP. We generated this antibody because we are studying if chicken can be infected with prions. In fact we have generated transgenic mice overexpressing chicken PrP.

Please, contact to me using [email protected] to discuss the details.