40 Questions 53 Answers 0 Followers
Questions related from Syed Zahid Ali Shah
Can anyone please give me a reference stating mitochondrial DNA copy number in prion diseases? Thanks in advance Ali
10 July 2017 487 0 View
i have a question related to Prion infection...i wanna know that vacuolation and neuron degeneration are in same region of brain or they can be two separate things? As i saw in some of my hamsters...
25 July 2016 6,242 4 View
i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...
26 April 2016 1,801 2 View
Can anyone tell me what are the fluorescent cells visible after thioflavin-S all around cerebellum of hamsters but they are only visible in the cerebellum of prion infected animals...in control...
22 April 2016 6,621 3 View
i want to detect caspase 9,12 and 3 in brain homogenates from hamsters..caspase 3 seem to be very difficult to detect via western blot? i am using 12% gell...any suggestions from people who work...
12 April 2016 8,184 6 View
i just want to know the accuracy of calcineurin antibody result and calcineurin colorimetric assay for brain tissue homogenates....and if i do western blot for calcineurin A and B subunits they...
13 March 2016 1,933 2 View
I want to detect BAD and pBAD (23 Kda size) and CREB and pCREB (43 Kda size) from brain samples of hamsters....When i do western blot with normal protocol giving 80 voltage for 4 hours to transfer...
06 March 2016 7,480 2 View
i wanna know if 263k strain can infect cell lines like N2a or HSSY5Y, if yes then can i use brain homogenate of infected hamsters to infect these cell lines? and what would be the best dose if i...
10 February 2016 4,778 2 View
i want to try effect of salubrinal as antioxident in embryo culture media but when i tried to put salubrinal first dissolved in dmso in m16 media it was not dissolved? what could be the reason?
24 January 2016 2,186 4 View
can anyone help me what brain homogenate concentration would be better if i want to detect Total CREB, pCREB, Total BAD and pBAD through WB from brain tissue of hamsters infected with Prion 263K?
15 January 2016 1,032 4 View
can anyone please help me how can i count activated astrocytes and microglia by using image j or any other software for statistical analysis? thanks in advance...
07 January 2016 5,235 6 View
i want to do calcineurin activity tests on brain homogenates, first i wanted to use an ELISA type of kit but i dont have ultrahigh centrifuge...so alternative could be if i use antibody for PP2B...
01 January 2016 6,151 3 View
i am doing experiment on brain homogenate from terminally dead syrian golden hamsters....i want to check Calcineurin level in homogenates...i will use Calbiochem cellular calcineurin activity...
29 December 2015 3,507 3 View
i have a very simple question that if i want to see the effect of Salubrinal and tauroursodeoxycholic acid (TUDC) in growth of reproductive cells for example in growth of embryo in vitro...will it...
01 December 2015 5,086 4 View
i just want to know if anyone knows the safe dose of minocycline that can be used in neuroblastoma cell line such as N2A? i did use in vivo but dont know much about invitro use...will be thankful...
18 November 2015 5,327 2 View
If I want to purify prion protein scrapie (PrpSc) from brain tissue of mouse and in the first step I need to put protease inhibitor cocktail in PBS, then homogenize brain in it. I have 100x...
18 November 2015 4,325 4 View
Do i need to pre heat the formalin fixed brain tissue slides before rehydration if i want to visualize prion plaques?
16 November 2015 4,106 5 View
it takes long time till the slides reach to room temperature after heat induced antigen retrieval so can i cool them down in refrigerator?
27 October 2015 2,737 4 View
If the primary antibody is raised in same species can I use multiple antibodies in same IHC slide? For example if I want to see neurons, astrocytes and microglia on same slide. All the antibodies...
02 October 2015 7,080 7 View
if i made ten percent brain homogenate and i want to add lysis buffer suppose RIPA buffer...what should be the ratio of PBS made homogenate to RIPA buffer? thanks
03 September 2015 8,844 2 View
if i want to detect Prpsc through WB, then i need to make brain homogenate and sonicate it first and centrifuged at 10K x g, but whether i will put PK after centrifugation or before? i personally...
13 August 2015 1,094 2 View
i wanna know if there is any good antibody that can detect prpsc in chicken?
21 July 2015 1,659 7 View
I want to know what is the incubation period after injecting 263k infected brain homogenate to Female syrian golden Hamsters? When do signs appears? what are first clinical signs? and when do they...
15 June 2015 2,836 5 View
I gave hamsters prion infection about 40 days ago. So with 263 strain signs should appear after approximately 60 plus days. Now, one of the hamsters is having movement problems in forelegs and it...
27 May 2015 9,711 3 View
i want to use rapamycin in my control group of hamsters infected with prion...whats the recommended dose? thanks
19 May 2015 5,930 2 View
i want to use 3 MA for stopping autophagy in my N2A transfected cell line overexpressing Parkin....but the 3MA that i bought shows on it 10 MIlimole/militre in DMSO? so how can i use it?any idea...
14 May 2015 5,926 7 View
i want to do an in vivo study related to prion neurodegeneration in syrian golden hamsters...during experiment i need to sacrifice animals for calcineurin activity...now my question is that i need...
29 April 2015 3,881 2 View
can anyone help me please if there is any kit available to detect calcineurin levels in brain of prion infected hamsters?
29 April 2015 7,871 1 View
Can I know if I can detect calcineurin levels in brain of hamsters after prion infection in fresh sacrificed animal brain only or is it possible to detect levels after the freezing of brain sample...
29 April 2015 10,055 1 View
If i want to use oxytetracycline or tetracycline hydrochloride in my syrian golden hamsters which route is safe for injections?and will it cross blood brain barrier? and will be thankful if...
22 April 2015 3,726 2 View
Can anyone suggest some Antibiotics that alters calcineurin levels in brain?or any drug that can change levels of pCREB and pBAD levels in brain?
17 April 2015 8,711 2 View
i want to detect stress produced by prion strain in SH-SY5Y or N2A but i am not sure which cell line is better?can anybody help me with selection of cell line?
13 March 2015 917 3 View
i want to see endoplasmic reticulum stress induced by PrPsc in SH-SY5Y cell line?any suggestions? which strain? any previous work? thanks in advance...
13 March 2015 6,766 1 View
i wanna know of any work done on SH-SY5Y cell line treated with any scrapie strain?
13 March 2015 9,774 3 View
When are proteins phagocytosed by the BV2 cells, is it necessary that they will be phagocytosed in lysosome? Or are they recycled to the plasma membrane?
12 March 2015 1,222 1 View
Does anybody know some papers about different concentrations of LPS stimulated macrophages that induce pro IL1 beta expression?
05 February 2015 1,045 4 View
I want to work on in vitro axonal degeneration caused by prion peptide 106-126. I want to confirm the best possible pathway of degeneration via western blot analysis. Can anyone help please?
23 November 2014 1,431 2 View
I want to know about calpain1 and calpain2 inhibition via calpastatin in vitro but i want to know more about calcium influx inhibition as a tool for axonal degeneration reversal mechanism?Possible...
19 November 2014 4,027 1 View
I want to know HDAC6 role in neurodegenerative diseases axonal transport caused by prion(prp106-126) in vitro
30 October 2014 8,715 3 View
In my lab we working on Prp scrapie 106-126 amino acid sequence.
09 October 2014 6,249 5 View
