DOes someone know what is the best basis set and method for calculating excited state of ion CH5+ ??

I've been trying to do my calculations related to optimization of excited state of ion CH5+ using method TD-DFT with M06-2X and aug-cc--pVTZ. My input file is as shown...

03 January 2021 2,148 0 View

Can I ignore the NMAR assumption when missing data is very negligible?

Hello! I am performing preliminary analyses on my data set (N= 187 heterosexual couples). When examining the summary of missing values, SPSS indicates that only 0.16% of my data is missing....

01 December 2020 5,562 13 View

Likert scale over repeated measures?

I am working on research where I have 66 participants who perform an exercise every day, and then they need to respond to 14 questions with a Likert scale (1-10). They performed this exercise for...

20 November 2020 4,682 6 View

Which anesthesia system should I buy to perform stereotaxic surgeries in mice?

I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many...

06 November 2020 3,613 3 View

Emission filter for FLIM to filter off GFP signal?

Dear all, I am measuring changes in fluorescence lifetime of my chromophore in cells expressing also GFP and I would need to filter off the GFP signal. After playing with the parameters in...

11 August 2019 5,297 3 View

Testing for correlation/regression between two scale scores on SPSS?

I am trying to find the correlation/regression between these two variables: Social media usage (measured by 10 items) (total scale score 50) Materialism (measured by 9 items) (total scale score...

10 April 2019 4,123 4 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

12 February 2019 5,959 2 View

Can dance/movement be a language? What are the effects of different languages on movement quality and understanding of movement?

I am researching whether dance can be considered a language, and different languages effects on movement quality. i am interested in how dancing to spoken word, interpreting it affects dancers...

14 August 2018 4,051 11 View

How to isolate DNA from water?

What is the best way to isolate genomic DNA from water? Using the ready kit centrifuge a sample of water to obtain the sediment of microorganisms or maybe filter water and isolate DNA from the...

07 August 2018 2,397 8 View

"Clouds" in 8.8 pH Trizma-HCl buffer?

Hi, I have found my Trizma-HCl buffer (strored in a fridge) to be contaminated with sth like "clouds" (see pictures). I prepared a fresh solution and during sdjusting the pH with NaOH (40% or...

07 January 2018 9,187 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View