I am trying to establish a control probe for RNA in situ hybridization for human samples. The plan is to synthesize DIG-labeled probes by in vitro transcription. Does anyone have primer sequences for cloning rDNA into the transcription vector?
if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...
24 February 2021 357 2 View
The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.
21 February 2021 4,733 19 View
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please
16 February 2021 8,801 3 View
i want to implement pseudo random number generator in matlab ...i request to every one if someone have code these PRNG share with me SplitMix Permuted Congruential Generator (PCG) Random...
14 February 2021 6,828 7 View
27 January 2021 4,815 5 View
What are the standard parameter values of the commonly used classifiers such as Support-vector machine, k-nearest neighbors, Decision tree, Random forest?
06 January 2021 6,940 7 View
Can anyone provide me the PDF for Distributed Energy Resources in Microgrids (Integration, Challenges and Optimization)?It would be really helpful
27 December 2020 8,015 3 View
Somepne please help me where to learn the simulation of Smart Grid on YouTube or other bookr esources?
22 December 2020 421 3 View
if the percentage of doping Au into rGO is too small 1~10%, is it not enough to prevent restacking of r-GO in xrd results, rGO peak appeared at 22.2 and also the peaks of gold nanoparticles so...
21 December 2020 6,091 3 View
is the concentration of gold salt that were added will affect on the peaks appeared. i added a very little amount of gold salt to dope graphene oxide and not all the peaks that proved the...
17 December 2020 9,275 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,
02 March 2021 0 0 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...
28 February 2021 3,442 2 View