I am trying to establish a control probe for RNA in situ hybridization for human samples. The plan is to synthesize DIG-labeled probes by in vitro transcription. Does anyone have primer sequences for cloning rDNA into the transcription vector?
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
The first step is to analyze a 2D molecular graph and implement partitioning techniques to calculate the topological indices. Secondly impose statically tools to generate QSPR model, may or may...
24 July 2024 9,644 1 View
Most of the time it is observed that in many manuscripts there are several typo error of topological calculation errors of calculation and partition of edges what ever the type of partitioning it...
22 July 2024 811 0 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
All I want to know is whether it is feasible or not. How did genes come to be if they do not come from parents?
12 July 2024 7,221 1 View
Pir Hassan Ali Shah live post, call for a question in your city about: What is the top problem, question, issue, add, advance, or share your point of view on subjects of money, economics, finance,...
09 July 2024 7,154 0 View
Pir Hassan Ali Shah post a question: How to do quantitative and qualitative data analysis after data collection? Give and review the answer in brief. All family members, friends, associates,...
08 July 2024 395 8 View
I am able to submit the code documents. Although my graph is 1, I would like to be in the 6E-05 graph range. Please, someone correct the error.
08 July 2024 5,770 0 View
Hello, ResearchGate community, I am currently exploring the Fuzzy Analytic Hierarchy Process (FAHP) methodology for a research project. I have a few questions that I hope the experts here can help...
02 July 2024 5,278 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View