Hello all,
I am currently trying to culture cortical neurons in media free of T3 in hopes to observe change when supplementing the cells with different concentrations of T3. I have looked up some literature and all I can seem to find is use of serum-free DMEM. Has anyone tried this before and can give me any insight on cell health? Or just any links to medias that can support neuron growth but also lack serum/T3?
Thanks in advance :)
Hello Jenna Thompson
You could refer to the link below. May be it would help you.
https://core.ac.uk/download/pdf/111035316.pdf
Best Wishes.
If you require T3-free media, perhaps you can formulate a custom serum-free supplement that omits it from the mixture. If you are going to use a lot of it, please reach out to our company, and we might be able to create a batch of our SM1 (our serum replacement supplement for neurons) that has T3 removed. Thanks.
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