Low carbon steels are being exposed to elevated temperatures for long time ,Is there any chance of carbides formation carbides ?

%C-0.16 %Si-0.13 %Mn-0.23 %S-0.009 %P-0.012 %Cr-0.024 %Ni-0.011 %Mo-0.006 %Al-0.031 Above composition is used for ducting material n the smelting furnace of high carbon ferro manganese as it is...

14 January 2020 1,013 2 View

What is the suitable etchant for 316 stainless steel for macro observation ?

Hello We have taken hot rolled and annealed stainless steel and forged it We are interested to find out its macro structure ? Please comment

21 April 2019 7,204 4 View

Is there any updated list of predatory journals/publishers available on the Web (like Jeffry Beall's)?

How one can find out the list of predatory journals/publishers (like Beall's list)?

11 March 2018 5,954 3 View

Is there a possibility to increase the benefit cost ratio (BC ratio) of the horticultural crops using fuzzy Logic?

09 February 2018 9,380 4 View

How can we confirm the phase of material through TEM diffraction pattern?

how can we confirm the phase of material through TEM diffraction pattern? If the calulated d-spacing is equal to the two phases d-spacing in JCPDF reference file?

20 June 2016 2,983 3 View

Is cloning a 9kb 3'UTR for luciferase assay necessary or cloning the regions of 3'UTR-miRNA interaction is sufficient?

08 February 2016 3,164 3 View

Is there any relation between coercive field and particle size and how can we calculate using Hc?

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01 May 2014 2,306 4 View

Is a discrete time system stable if and only if all the roots are located inside the unit circle z<<1?

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21 February 2014 1,651 9 View

Is there any good protein protein interaction prediction software or tools?

Kindly suggest me

25 January 2012 2,953 17 View

How reliable is the usage of Xylose lysine deoxycholate(XLD) agar for the detection of E.Coli in food samples?

Primarily the usage of XLD agar is for the detection of Salmonella species in food analysis. Some sources say that e.coli also can be detected by the presence of yellow colonies. Theoretically...

01 January 1970 9,074 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View