Dear Naveen,

I am sorry to be unable to provide a direct answer, but its a quite difficult question.

It depends on many factors:

1) Is the compound supposed to be cytotoxic or non-toxic?

2) Is there literature around the compound?

3) Is there a great difference between replicates? Have you already performed biological replicates?

4) Are you using negative and positive controls? Are the results consistent?

5) Have you done a dose-response test, with many different doses, so you can see if the results are dose-dependent?

6) Have you performed additional analysis, such as Western Blots, qPCRs, cell proliferation assays, or any other thing that might show by which mechanisms your compound is acting?

Hope this can bring some light to the matter,

Best regards

In examining the effect of a compound or medicine, a concentration of a substance in which 50% of cells are alive and 50% are dead is called IC50.