At what temperature the enzymes that split nonstructural carbohydrates in plants are killed?

29 October 2020 6,410 4 View

Finger tapping - which sensor?

We are developing a markerless software for the measurement of bradykinesia (finger tapping) using two monochromatic IR cameras and three infrared LEDs. We would like to validate the results with...

19 October 2020 6,819 2 View

Boiling temperature of 50% ethanol at 6000 m a.s.l.?

18 October 2020 7,718 4 View

What kit use for bacterial dna extraction?

Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...

30 July 2020 6,566 7 View

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)?

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)? Especially, not using Santa-Cruz antibodies? I am being...

04 June 2018 3,394 3 View

Can I detect autophagy via FACS with extracellular staining?

I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...

09 July 2017 1,116 3 View

Hello everybody! Does anyone have experience with isolating bacterial DNA from skin swabs?

23 July 2015 4,748 7 View

Can anybody help with separation of genomic and viral episomal DNA?

I want to get pure genomic fraction and pure episomal fraction without cross-contamination. I want to use them in PCR. I am working with herpesvirus which has quite a large genome. Any suggestions?

09 April 2014 5,111 7 View

How to increase tissue macrophages

Hello, I am currently in a desperate need of a group brainstorming and I thought I could use your help. I would like to develop an experimental model of human lung cancer and the aim is to test...

01 January 1970 164 1 View

Measuring the protease activity

Hi everybody, does anybody have reliable protocol for measuring the protease activity? We would like to establish the protease activity from laboratory compost experiment (made from sawdust) and...

01 January 1970 7,620 9 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View