At what temperature the enzymes that split nonstructural carbohydrates in plants are killed?

29 October 2020 6,410 4 View

Finger tapping - which sensor?

We are developing a markerless software for the measurement of bradykinesia (finger tapping) using two monochromatic IR cameras and three infrared LEDs. We would like to validate the results with...

19 October 2020 6,819 2 View

Boiling temperature of 50% ethanol at 6000 m a.s.l.?

18 October 2020 7,718 4 View

What kit use for bacterial dna extraction?

Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...

30 July 2020 6,566 7 View

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)?

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)? Especially, not using Santa-Cruz antibodies? I am being...

04 June 2018 3,394 3 View

Can I detect autophagy via FACS with extracellular staining?

I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...

09 July 2017 1,116 3 View

Hello everybody! Does anyone have experience with isolating bacterial DNA from skin swabs?

23 July 2015 4,748 7 View

Can anybody help with separation of genomic and viral episomal DNA?

I want to get pure genomic fraction and pure episomal fraction without cross-contamination. I want to use them in PCR. I am working with herpesvirus which has quite a large genome. Any suggestions?

09 April 2014 5,111 7 View

Does anyone have experience with separation monosaccharides (neutral and acid) on the Dionex columns?

Which column (CaerboPac MA1, PA1, PA100) is the best? Is it possible to detect neutral and acid monosacchairdes on the one column?

20 September 2013 4,314 1 View

How to increase tissue macrophages

Hello, I am currently in a desperate need of a group brainstorming and I thought I could use your help. I would like to develop an experimental model of human lung cancer and the aim is to test...

01 January 1970 164 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

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17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View