At what temperature the enzymes that split nonstructural carbohydrates in plants are killed?

29 October 2020 6,410 4 View

Finger tapping - which sensor?

We are developing a markerless software for the measurement of bradykinesia (finger tapping) using two monochromatic IR cameras and three infrared LEDs. We would like to validate the results with...

19 October 2020 6,819 2 View

Boiling temperature of 50% ethanol at 6000 m a.s.l.?

18 October 2020 7,718 4 View

What kit use for bacterial dna extraction?

Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...

30 July 2020 6,566 7 View

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)?

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)? Especially, not using Santa-Cruz antibodies? I am being...

04 June 2018 3,394 3 View

Can I detect autophagy via FACS with extracellular staining?

I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...

09 July 2017 1,116 3 View

Hello everybody! Does anyone have experience with isolating bacterial DNA from skin swabs?

23 July 2015 4,748 7 View

Can anybody help with separation of genomic and viral episomal DNA?

I want to get pure genomic fraction and pure episomal fraction without cross-contamination. I want to use them in PCR. I am working with herpesvirus which has quite a large genome. Any suggestions?

09 April 2014 5,111 7 View

Does anyone have experience with separation monosaccharides (neutral and acid) on the Dionex columns?

Which column (CaerboPac MA1, PA1, PA100) is the best? Is it possible to detect neutral and acid monosacchairdes on the one column?

20 September 2013 4,314 1 View

How to increase tissue macrophages

Hello, I am currently in a desperate need of a group brainstorming and I thought I could use your help. I would like to develop an experimental model of human lung cancer and the aim is to test...

01 January 1970 164 1 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View