I looked for papers but couldn't find useful ones.
I would like to prepare a stock, aliquot and freeze, since the lyophilized powder from Sigma is only 2 mg and I can't weight less than ~ 0.5-1 mg accurately.
Also, which are the optimal condition for this enzyme? Do you diluted on water or buffer? Time/temp of incubation? I'm working in a protocol for extraction of DNA from S. aureus from clinical samples.
Thank you!
Having no idea, which specific Lysostaphin you are using from MilliporeSigma, I can help you, more specifically. I have attached the Product Information sheet for L7386 Lysostaphin that basically does not recommend that the material be frozen after reconstituted, however it contains references that show some applications of using a material that was frozen.
Vernon L Reisenbichler Thank you for answer.
The cat# SAE0091.
I see the specifications on this product but it doesn't explain much of how to do it.
If you can help me, I will very grateful for your advice.
Best,
Laureana
Preparation Instructions The product is soluble in water (10 mg/mL), yielding a clear to slightly hazy solution. We recommended that fresh solutions be prepared, as the product loses activity in solution. We do not specifically recommend the freezing of lysostaphin solutions in aliquots. One published reference cites storage of 200 units/mL stock solutions of lysostaphin in a buffer of 0.05 M Tris-HCl and 0.145 M NaCl at pH 7.4. 7 A second reference indicates storage of 2 mg/mL stock solutions in 20 mM sodium acetate buffer, pH 4.5, in frozen aliquots.8 However, we have not tested either situation ourselves. Storage/Stability Store the product at –20 C. When stored properly and unopened at –20 C, the enzyme has a recommended retest date of 3 years.
- Badges
- Science method