Hello,
I have ABTS that in the past I used to measure reductive activity. Now I want to measure oxidase activity. I was wondering if I could use the ABTS as a substrate for this experiment.
I will purify a protein that I think can oxidase compounds and incubated with ABTS, while measuring the change of colour in the medium.
Thank you Nick Gee . Do you have any recommendation literature to see the protocol for that?
yes you are absolutely right Nick Gee ! I have to check my conditions because I have been reading and, usually the oxidation of ABTS is measure under acidic pH (4.5-5). However, I do not know if my protein will tolerate that.
Yes, the literature will be dominated by HRP assays and hence pH values around pH 5 +/- 0.5 units. However, I've certainly seen pH activity studies done with this substrate in the range pH 4-7. Hopefully someone with experience of enzymes other than HRP will be able to comment about potential use of ABTS under slightly alkaline conditions.
Excellent. I will certainly try a range of pH to see if I have any differences. Thank you for taking the time to comment on this.
Absolutely, ABTS is use for oxidation process. i used ABTS to measure the enzyme's activity (laccase in my study). You can read some of this paper for further references.
- DOI:10.3303/CET2078007
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