How to compute the distance in km between two points inside the Earth?

Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...

02 February 2021 3,455 4 View

How can I project 3D objects on 2D images??

Hello! From 3D images stacks I have recovered some objects. Now I need to project those objects on 2D for further analysis. Any idea, any software, plugin, etc?

21 January 2020 4,144 4 View

Does anybody knows if there is a metodology of acute ethanol toxicity for neuroprotection studies that uses functional foods?

I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...

03 January 2020 9,709 2 View

Is there a way to normalize my western blot bands since i loaded equal volume of seminal plasma (not based on protein concentration)?

I loaded 10ul of raw seminal plasma sample + 10 ul Bolt LDS buffer +4ul Bolt reducing reagent. I did the transfer using iBlot2 dry transfer system and used One Pierce ECL protein detection kit....

06 May 2019 6,912 3 View

PArticipate in my Ph.D research?

31 December 2018 3,283 0 View

Is there an acceptance criteria or limit for swelling index/behavior for sustained release matrix tablets?

I am doing a project on Matrix Tablets using natural products as binders (release retardant polymer) and I came across Swelling Behavior/Index. Is this necessary? I know swelling is vital for gel...

03 December 2017 8,272 2 View

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy?

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy? Solution A is pink while Solution B is green in color. If I will mix the...

16 October 2017 6,761 3 View

In identifying microorganisms using BLAST search, what is the purpose of converting the reverse sequences to reverse complement?

I have a 27F and 1492 R primers. And I wonder why the R primers should be converted to its reverse complement.

03 August 2017 6,530 2 View

Why I have low reproducibility in my real time pcr?

Hello , I'm quantifying bacteria by real- time PCR . I use plasmids with the target sequence as standards. The reproducibility is not good, one time i have 95% of efficiency and the next day is...

18 July 2016 4,605 7 View

How I can know the degree of crosslinking of a polymer?

Is it known that there is a variation in the degree of crosslinking varying synthesis parameters, but that technique I can use to know this degree?

28 April 2015 3,119 6 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View