Do you know who can supply us the Costa Rican coral snake venom?

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Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Is there a validated questionnaire for target audience research?

Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...

04 July 2024 9,001 0 View

Posting a research survey ?

Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...

30 June 2024 9,594 2 View

Can I use richer media to increase bacterial expression of human protein?

We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?

06 June 2024 6,071 1 View

How to scale up or down a restriction enzyme reaction?

Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...

28 May 2024 1,105 3 View

Algae contamination on hydroponic cultures for Arabidopsis thaliana?

Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...

21 May 2024 4,840 1 View

How to set up a restriction enzyme reaction?

I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...

19 May 2024 5,171 3 View

What is recommended as a strong and current reference of phenomenological analysis ?

What is recommended as a strong and current reference of qualitative phenomenological analysis ?

16 May 2024 2,142 1 View

Is there any guide to perform statistical analysis of transcriptomics?

I am looking for a some chapter or book related to the statistical analysis of mRNA transcriptomics. Thank you;)

09 May 2024 6,543 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View

How can rhizobial strains be evaluated without plant experiments?

Hi everyone, I'm looking for a method to evaluate the nodulation ability and effectiveness of different strains of rhizobia. Is there any method that avoids testing in plants? Are there any...

17 July 2024 7,249 0 View

Phengaris rebeli on Picea abies?

Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...

15 July 2024 8,099 4 View

Any suitable Animal models to evaluate Mast cell stabilizers?

I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.

14 July 2024 779 0 View

Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Russell Eyre

A few decades ago, the Miami Serpentarium would have likely been able o help. I'm not sure the status of the business now.

Laura Feldman

Thanks for your kindly reply

Benedito Barraviera

Hi Laura. No, we do not have these venoms. Benedito

Sergey Kolesnikov

No, I am not a specialist in this field