After Preparation of Liposomes-containing Bevacizumab using dehydration-rehydration method , sample was centrifuged,then concentration of avastin in supernatant was measured with BCA method.
Strangely the amount of protein in supernatant is a bit higher than tatal protein. So encapsulation efficiency (EE%) is negative! Do you think it is because of Experimental error or there is something else in reaction that has aborbance?
thx
Dear Maryam,
I have not tried it with the specific antibody you mention, I would suggest trying a liposome extruder to encapsulate the solition. Suggest using something like this:
http://www.avestin.com/lf.html
Relatively simple and relatively inexpensive.
Kind regards
Rob
did u prepared standard curve or equivalent absorption of pure drugs ?
Its most likely possible that excipients of liposome are also exhibiting absorbance at same wavelength.
partially miscibility exhibiting same issues there.
Did u measure free drugs concentration to calculate encapsulated one. I f not then try for same too.
I found that bradford and BCA are not suitlable for my goal. They interfere with lipid. so results seems weird.
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