Hi,

I am working with some ILs and have some great dates, but still I found some problems. I was testing those ILs on bacteria to know how they influence on them and on degradation of some organic compounds, but I have problems with bacteria aggregation. In some cases strains are aggregating to each other, but still alive. I checked it by using two dyes: PI and orange. the data are confusing because during tests with dehydrogenase the results are quite different.

Does anyone know how I can check if why bacteria are aggregating even with small concentration of ILs and how to interpret those kind of results? It seems that some of ILs are very toxic even in low concentration, but there are different date for Gram + and Gram - bacteria. Gram - bacteria are more immune for the ILs.

Thanks for help.

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