Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

Machine Learning and Big Data Analytics Tools Question?

What would be the best big data analytical tools to determine changes in behavioral health (e.g., addiction behaviors)? The data used to predict behavioral health changes are secondary data which...

30 September 2020 9,522 8 View

What is a good rice variety to research on red, brown, black and white rice towards antioxidant properties and anti-alphaglucosidase on diabetes?

We will be conducting research on colored rice--red, brown, black and white rice and utilize FRAP, superoxide assay and hydroxyl scavenging assay to determine antioxidant activity and correlate it...

20 April 2020 7,334 3 View

Efficient preps for chunky human adipose tissue?

Hi all, I aim to isolate SVF from the tissue. However, we sometimes receive big chunks of tissue that is not through liposuction. I've been wondering is there a ingenious way to efficiently prep...

24 February 2020 6,104 1 View

What is the difference Knee joint Cryo section vs Paraffin section?

I have a tissue that causes arthritis with MIA. Usually everyone uses Cryo or Paraffin section for knee joint. What's the difference?

23 December 2019 8,103 5 View

Why does my starting culture for maxiprep not able to grow?

I have my plasmid (pCMV-4 backbone) in a DH5alpha cells and I have spread it on a LB+AMP plates (final conc of AMP= 0.1mg/ml). These plates have colonies that grew well with the expected shape and...

16 August 2019 2,866 1 View

Recovered Carbon Black from Waste Tire Pyrolysis: How to estimate the level of odor of recovered carbon black (due to VOC)?

Dear All, Currently, we are facing quality issue of our recovered carbon black(rCB) that our customer complained our rCB got unpleasant smell. I suspected those are due to volatile matter...

05 August 2019 9,657 2 View

Coculture of T cells and Osteoclast precursors?

Hi, I am trying to set a co-culture system of T cells from immunenized mice and mcsf treated mouse bone marrow. However, it is not successful to induce osteoclasts. I read that we can fix...

24 July 2019 7,157 5 View

Why are the silver used with a little ruthenium or gold for a metallic mirror?

I heard that metallic mirror is made by silver or aluminum having high reflectance. In this case, usually Ag deposited with Ru. Alloy or stability against oxygen?

15 July 2019 6,625 2 View

How to visualise the trace of a atom from an AIMD calculation (Vasp + Quantum Espresso) ?

Dear All, As mentioned in the title I want to trace the dynamic of an atom from an AIMD trajectory. Is there any tool to visualise it ?. I want something like the attached figure. Regards Nam Tran

20 March 2019 8,976 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View