MTORC1 activation marker: S6K or 4EBP-1 ?

More Nam Leba's questions See All

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

Efficient preps for chunky human adipose tissue?

Hi all, I aim to isolate SVF from the tissue. However, we sometimes receive big chunks of tissue that is not through liposuction. I've been wondering is there a ingenious way to efficiently prep...

24 February 2020 6,104 1 View

What is the difference Knee joint Cryo section vs Paraffin section?

I have a tissue that causes arthritis with MIA. Usually everyone uses Cryo or Paraffin section for knee joint. What's the difference?

23 December 2019 8,103 5 View

Coculture of T cells and Osteoclast precursors?

Hi, I am trying to set a co-culture system of T cells from immunenized mice and mcsf treated mouse bone marrow. However, it is not successful to induce osteoclasts. I read that we can fix...

24 July 2019 7,157 5 View

Why are the silver used with a little ruthenium or gold for a metallic mirror?

I heard that metallic mirror is made by silver or aluminum having high reflectance. In this case, usually Ag deposited with Ru. Alloy or stability against oxygen?

15 July 2019 6,625 2 View

How to visualise the trace of a atom from an AIMD calculation (Vasp + Quantum Espresso) ?

Dear All, As mentioned in the title I want to trace the dynamic of an atom from an AIMD trajectory. Is there any tool to visualise it ?. I want something like the attached figure. Regards Nam Tran

20 March 2019 8,976 2 View

Chain rule on Caputo fractional derivative?

In formula 16 of the paper called "Fractional-order gradient descent learning of BP neural networks with Caputo derivative". (I attached the paper below) The single variable chain rule for the...

30 January 2019 5,518 1 View

Genomic DNA requirement for genotyping using SNPType assays (Fluidigm)?

Hello, I am working with pumpkin genotyping using SNPType assays (Fluidigm). My gDNA samples have 260/280 ratio in a range of 1.7 - 2.0 and 260/230 ratio in a range of 1.0 - 1.8. I am wondering...

08 July 2018 4,427 2 View

What further information is needed inside the Abaqus to match the experimental load-deflection curve?

I'm trying to simulate the tensile test of corroded tensile specimen and compare the results from the experiment which is already done. I tried to extract the reaction force at the reference...

22 May 2018 2,317 4 View

Does the abundance of a mature miRNA always positively correlate with that of its pri/pre miRNA?

Hi there, I am working on plant miRNAs using Next-Generation-Sequencing data. I have found in literature that some studies indicate a high correlation between expression of mature miRNA and its...

10 April 2018 8,312 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

Yong Chen

In my case, these 2 substrates are always consistent. It's not a good idea to pick data. I think it is interesting to find something "strange". You may make a great story! Good luck!

Nam Leba

Yong Chen thank you for reminding me to view from another aspect. I will do more research about that.