21 July 2023 1 6K Report

I would like to find out the amino acid sequence for a few CD3 antibodies. Does anyone also know any platform/database that I can find amino acid sequence of antibodies?

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Why are bovine satellite cells differentiated at 30% confluency?

Hi, I have been culturing bovine digest satellite cells recently. In my very latest study, i seeded it at 2.65 million cell in T75 with dmem/f12+20% fbs and 1% antian and 10ng/mL fgf and tried...

24 July 2023 9,514 0 View

Cell lysates from normal human epidermal keratinocytes, adult (NHEK) for keratin filaments quantification?

Greetings fellow researchers. I am doing experimental work on normal human epidermal keratinocytes, adult (NHEK) monolayer cell culture and I am interested to analyse the proliferation and...

17 July 2023 6,270 0 View

What is limitation of electrorotation for objects with nanometer size?

Eletrorotation was used for the measurement of electrical property of cells like dielectric constant and there were also some works for the electrorotation of nanowire, but the length was still...

14 March 2023 1,489 0 View

G2/M peak is far from G0 peak in cell cycle analysis?

Hello everyone. I have performed cell cycle analysis by FCM using FxCycle PI/RNase staining solution from Invitrogen for MSCs. I fixed my cells with 70% ethanol for 2 hours at 4 degree C before...

02 February 2023 2,302 2 View

How to avoid the MHD activities during the plasma current ramp up phase in TOKAMAK?

We know the MHD such as the NTM ,Kink mode would affect the confinement or the transport of the plasma in TOKAMAK.They have different driven free energy but not only that.In general, the MHD will...

28 August 2022 3,023 1 View

What is the proper procedure to produce Fatty Acid Chloride by using Palmitic Acid and Thionyl Chloride?

I was entrusted with the project to find out the experimental procedure of producing Fatty Acid Chloride using Palmitic Acid and Thionyl Chloride, mostly the resources online are general so I need...

01 March 2022 1,927 2 View

Why is there a difference in BCA mitochondrial protein quantification results using DPBS and storage buffer?

I isolated mitochondria using Sigma-Alrich Mitochondria Isolation kit for Cultured cells (MITOISO2) and resuspended the mitochondria pellet using their Storage Buffer (50 mM HEPES, pH 7.5,...

11 February 2022 8,392 4 View

What's your thoughts on ArcGIS Pro v2.9 accuracy assessment tool?

Hi everyone, I would like to ask for your opinion about the accuracy assessment tool on ArcGIS Pro. I am currently using the software to run drone image classification. I have done the accuracy...

10 February 2022 2,178 0 View

Does anyone has MATLAB code for 1D discrete nonlinear Schrodinger equation evolution?

I have tried the ODE method, but the effect is not ideal.

12 January 2022 1,155 3 View

Machine Learning and Big Data Analytics Tools Question?

What would be the best big data analytical tools to determine changes in behavioral health (e.g., addiction behaviors)? The data used to predict behavioral health changes are secondary data which...

30 September 2020 9,684 8 View

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

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Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

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The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

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How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How to convert g/kg Humic acid dose to kg/ha?

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How to make a Lateral flow control line more uniform?

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