Does anyone have a good protocol for COMET assay for plant root tissue?
Although a few years ago, we had some nice results with the COMET assay using the protocol described in http://www.ncbi.nlm.nih.gov/pubmed/?term=Parlesak+Comet.
HAve success, Alex
The protocol used by myself recently.
Thanks guys. I plan on doing the COMET assay soon. Will keep guys in touch.
Plasmid size:40kb E.coli strain: DH5 alpha I have tried using Qiagen midi prep plus kit with no luck. I need at least 100ng/ul concentration for sequencing. I have also tried the routine manual...
10 November 2014 4,500 19 View
Hi I was wondering if there is any online software to check the Tm specifically for PCR product?
09 October 2014 1,470 16 View
I have more than 300 plants to genotype. I am trying to find something quick which can be done in 96 well or any protocol which is feasible for large number of plants. I plan on performing PCR...
08 September 2014 4,980 8 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View