Preetam Janakirama

4 Questions 9 Answers 0 Followers

Questions related from Preetam Janakirama

Does anyone know a good/efficeint protocol for extracting a low copy number plasmid from Ecoli?

Plasmid size:40kb E.coli strain: DH5 alpha I have tried using Qiagen midi prep plus kit with no luck. I need at least 100ng/ul concentration for sequencing. I have also tried the routine manual...

11 November 2014 4,473 19 View

Does anyone know a on-line software to check Tm (melting temperature) for PCR product?

Hi I was wondering if there is any online software to check the Tm specifically for PCR product?

10 October 2014 1,448 16 View

Can someone suggest me a quick and a good protocol for plant DNA extraction please?

I have more than 300 plants to genotype. I am trying to find something quick which can be done in 96 well or any protocol which is feasible for large number of plants. I plan on performing PCR...

09 September 2014 4,958 8 View

Does anyone have experience on COMET assays?

Does anyone have a good protocol for COMET assay for plant root tissue?

08 August 2014 7,770 3 View