According to ATCC ..."the myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent, and the line should be recloned periodically with selection for myoblastic cells".
Could you please let me know how to select and differentiate the myoblastic cells from others?
Hello
Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation.
to cultuer this cells please use the following recommended media DMEM with 4mM Glutamine and 1.5g/L NaHCO3 with 10% FCS and Pen/Strep or Gentamycin. They will be 70% confluent on the third day. Split them at 70% confluency, do not let them grow more than 70 - 80% confluent.
I hope this works for you , Good Luck
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