Does anyone have CD248 (endosialin, TEM1) anti-mouse antibody for FACS?

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Russell Linscott

We list a couple of sources with Rabbit pAbs vs CD248, which they claim cross-react with human, mouse and rat, and are suitable for FC. Search here http://www.linscottsdirectory.com/search/antibodies for CD248 and select "mouse" and "Flow Cytometry" from the Reactive Species and Applications filters. Hope this helps.

Sabine Strehl

Has anybody used a CD248 anti-HUMAN antibody for FACS and can recommend one? BD has an Alexa Fluor® 647 Mouse Anti-Human Endosialin (CD248) Clone B1/35 antibody? Has anybody tested this one?

Simon Molgaard

Hi Desiree and Sabine.

I hope you will also share your experience with the antibodies on www.pAbmAbs.com? Here, scientists can share information about the antibodies they use in their research. So far, noone has reviewed antibodies against CD248, so your input is therefore very valuable for other scientist when they are looking for reliable antibodies.

As a bonus a can let you know that all reviews received before dec 15th will enter a competition where the winner will receive €500 for personal spending. Every review counts as a lottery ticket-also reviews on antibodies that have already been reviewed by other scientists before you. It is quite simple to review. All you have to do is go to the website, click "submit review" and here it is described how to design the review.

Kind regards,

Simon

Desiree Straign

Hi Simon,

I will keep that in mind. I have not used the CD248 antibody because I would have to conjugate it myself and also purchase a lot of the purified antibody to do so. (Also, the specificity was less than 85% for mouse).

Best regards,

Desiree

Hi Desiree.

Sounds good! You are of course also welcome to rate other antibodies you have used. The more reviews the better!