What structures are essential for the development of a K-12 Learning design team? Please share ideas of lesson plans and content organisation?

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How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

Path analysis: Do I control for covariate effects on all endogenous variables (and mediator) or just DV?

I did research and the studies suggest controlling for the effects of covariates on all endogenous variables, including mediator variables, to improve model accuracy and reliability. I want to...

04 June 2024 1,914 3 View

Suggestions for a low-cost, small-scale germination chamber?

I want to conduct a small seed germination experiment and am looking for advice on incubation instruments. My neaby colleagues don't have any, and the costs of lab-standard plant growth chambers...

15 March 2024 3,752 0 View

What native antibody is found in Het3 mice?

I am trying to determine if Het3 mice produce antibody to a compound. This compound should be non-immunogenic. They have not been exposed to anything else. Apart from injecting mice with an...

18 February 2024 5,271 0 View

Literature: how do energy interests use energy crises?

I am looking for literature on how different interests (e.g. energy companies, governments, political parties, industries, business associatons, etc) use energy crises and concerns over energy...

31 January 2024 4,248 0 View

How long can you store MitoSox after it has been reconstituted?

Hello, I am not needing to use the full 10uL of MitoSox at one time. Per Thermo tech support, the unused portion can be aliquoted and stored at -20ºC after perfusing with nitrogen. Though they do...

21 November 2023 7,016 0 View

Tol2 - Is it normal to lose the transgene expression after a few days?

I'm using a transposon tol2 vector (pT2AL200R150G - 10.1534/genetics.106.060244) to create transgenic zebrafish line. We first inserted our gene of interest (GOI) into the BamHI site of the...

21 November 2023 9,618 1 View

Where can I find the raw data for S-N curve of steels?

Dear fellow researchers, I would like to know where I can find the editable/raw data for a set of points of a S-N curve for a high strenght steel, like a dual phase. I can only find the S-N...

19 November 2023 8,866 3 View

[Microstrip Combline Filter Design] - How do I choose the resonator's admittance, Y_{ai}, for optimal Q?

Matthaei (et al) writes that a common choice is Y_{ai} = 1/70; I've seen ranges from 1/60 to 1/100. Please could someone provide some intuition on the choice for Y_{ai} in Matthaei's design...

09 November 2023 5,741 1 View

How do I calculate the even and odd mode impedances of coupled microstrip lines in CST?

I'm trying to determine the spacing between resonators for a planar bandpass combline filter, such that the even and odd mode impedances match those that I've calculated as part of the filter...

05 August 2023 3,005 3 View

Corporate Social responsibility strategy?

strategy and organisation of effective social responsibility in corporations

24 July 2024 5,003 2 View

How the KwaZulu natal riot affected south Africa?

How the damages of building and shopping centers affected the south African economy?

24 July 2024 5,400 2 View

What are the factors that hinder a high performance culture?

What are the specific factors that hinder implementation of a high performance culture and what are the mechanisms that could be employed to support implementation. These factors could be at...

20 July 2024 8,529 2 View

Formation of a target organizational and functional model for data management in space activities?

Formation of a target organizational and functional model for data management in space activities

10 July 2024 4,498 0 View

Given the organizational complexity of academic institutions does an internal institutional politics play significant role in an institution's growth?

There are few business activities more prone to a credibility gap than the way in which executives approach organizational life. A sense of disbelief occurs when managers purport to make decisions...

08 July 2024 1,323 2 View

Coulkd you help finding a paper about Bryophytes ?

Some monthes ago on RG I found a nice paper by a bryologist explaining that in a single small tuff of epiphytic mosses different species might co-occur. I remember a plate illustrating type...

01 July 2024 5,102 0 View

Is there a unified register of aircraft maintenance organisations?

I am currently working on a study on aircraft maintenance. I am interested in organisations that perform maintenance. Are there any special registers that contain information on such...

26 June 2024 7,734 0 View

What is the position of the International Court of Justice on the request of the Republic of South Africa regarding the crimes in Gaza?

24 June 2024 6,148 4 View

Is really exist postoperative infections after treatment of upper limb fractures in precarious environment?

Has anyone in Sub-Saharan Africa or South Asia ever encountered an unusual increase in the rate of surgical site infection following internal fixation of closed upper limb fractures?

24 June 2024 2,848 1 View

Finding Survey Participants?

Dear Survey Experts, I am currently researching trust and resilience in the supply chain and seeking efficient ways to find survey respondents. In addition to social media and business...

21 June 2024 395 2 View