Hey,
I´m struggling with the results of the two methods.
I use the Bioanalyzer (RNA 6000 Pico Kit) and Nanodrop to measure the concentration and integrity of my mRNA samples. I heat denature the samples before measurement as recommended in the instructions.
Concerningly, I find my Nanodrop concentrations to be about 4 times higher than my Bioanalyzer concentrations.
Within the method there is not much of a difference, the reproduceability seems good.
There samples contain lipids, therefore we did a purification step (RNeasy Plus Microkit) leading to an immense loss of concentration on both methods. Nanodrop was still 2 times higher.
Does anyone know by any chance why there is such a difference?
Moreover, does anyone know what excactly is the conditioning solution for on the Bioanalyzer? I can`t find any informations about this.
Thanks