29 March 2023 1 655 Report

Hey,

I´m struggling with the results of the two methods.

I use the Bioanalyzer (RNA 6000 Pico Kit) and Nanodrop to measure the concentration and integrity of my mRNA samples. I heat denature the samples before measurement as recommended in the instructions.

Concerningly, I find my Nanodrop concentrations to be about 4 times higher than my Bioanalyzer concentrations.

Within the method there is not much of a difference, the reproduceability seems good.

There samples contain lipids, therefore we did a purification step (RNeasy Plus Microkit) leading to an immense loss of concentration on both methods. Nanodrop was still 2 times higher.

Does anyone know by any chance why there is such a difference?

Moreover, does anyone know what excactly is the conditioning solution for on the Bioanalyzer? I can`t find any informations about this.

Thanks

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