Farell & Carter (2019) state that visual inspection for changes in serum or plasma color and clarity has traditionally been used to determine and quantify HIL interference. Any turbidity, redness, or yellowness was rated subjectively using the HIL index. Aside from the visual inspection of the samples, are there any other methods to determine and quantify HIL interferences present in the sample?
Reference:
Farell, C. J. L., & Carter, A. C. (2019). Serum indices: Managing assay interference . SAGE Journals. Retrieved February 23, 2023, from https://journals.sagepub.com/doi/10.1177/0004563216643557
Hemolytic samples can be measured photometrically (by measuring free hemoglobin) and can be prevented by having systems and recommendations in place.
Icteric samples can be identified photometrically at an absorbance of 480 nm and 505 nm. Resolving icterus can be accomplished by sample dilution to reduce icterus below the interference threshold.
Lipemic samples is most often detected indirectly as a measure of sample turbidity at a light absorbance of 570 nm and 660 nm. We can resolve this inference by sample ultracentrifugation.
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