Does anyone have any experience with measuring AP-1 activation?

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Has anyone got a measurement that could be taken to ensure that an intranasal dose of LPS has been administered successfully?

Dear all, I've been doing some experiments using intranasal dosing of LPS, a technique that has been well described in the literature and I achieve very uniform results (on the whole)....

09 October 2014 4,043 10 View

Anyone know a good IL-1RA antibody for flow cytometry for murine cells?

Hi all, I'm trying to look at IL-1ra expression in lung injury but I'm struggling to find an antibody. Does anyone have any suggestions? Thanks

02 March 2014 9,506 2 View

Any suggestions on a commercially available culture vessel for macrophages?

I used to use PFTE coated bags that were then sterilised with gamma irradiation for macrophage culture. This worked very well but I have since moved university and the bags were an in house thing...

31 December 2013 8,230 4 View

Extraction of alveolar macrophages?

Does anyone have any experience with the recovery of alveolar macrophages? We are struggling to get a decent yield because as soon as we infuse saline or media the macrophages seem to adhere very...

10 November 2013 6,905 29 View

Does anybody have any experience assaying Arginase-1 activity in Macrophages?

I'm planning to do some M1 vs M2 macrophage work and am working out the best way to distinguish these subsets. I know you can try this via flow cytometry, however I'm yet to find a suitable Arg-1...

06 July 2013 4,002 16 View

What markers would people recommend when investigating microglial activation by flow cytometry?

I'm currently doing some work with the BV-2 microglial cell line and I'm investigating the effects of a drug on their activation after LPS stimulation. Does anyone have any advice about what some...

04 May 2013 9,836 17 View

Has anybody got any experience with cell cycle synchronisation in either T cells or a microglial cell line?

I can find protocols for HeLa cells, however, we want to synchronise either T cells or monocytes to image them following drug treatment. We're aiming to determine whether the drug causes spindle...

03 April 2013 2,400 20 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View