8 Questions 34 Answers 0 Followers
Questions related from Charly Oakley
Dear all, I've been doing some experiments using intranasal dosing of LPS, a technique that has been well described in the literature and I achieve very uniform results (on the whole)....
10 October 2014 4,102 10 View
Hi all, I'm trying to look at IL-1ra expression in lung injury but I'm struggling to find an antibody. Does anyone have any suggestions? Thanks
03 March 2014 9,574 2 View
I'm looking to study AP-1 activation in some in vivo models of lung injury and was wondering whether anyone has any experience in doing this? Preferably I'd like to study it via flow cytometry but...
02 February 2014 1,258 3 View
I used to use PFTE coated bags that were then sterilised with gamma irradiation for macrophage culture. This worked very well but I have since moved university and the bags were an in house thing...
01 January 2014 8,290 4 View
Does anyone have any experience with the recovery of alveolar macrophages? We are struggling to get a decent yield because as soon as we infuse saline or media the macrophages seem to adhere very...
11 November 2013 6,966 29 View
I'm planning to do some M1 vs M2 macrophage work and am working out the best way to distinguish these subsets. I know you can try this via flow cytometry, however I'm yet to find a suitable Arg-1...
07 July 2013 4,068 16 View
I'm currently doing some work with the BV-2 microglial cell line and I'm investigating the effects of a drug on their activation after LPS stimulation. Does anyone have any advice about what some...
05 May 2013 9,903 17 View
I can find protocols for HeLa cells, however, we want to synchronise either T cells or monocytes to image them following drug treatment. We're aiming to determine whether the drug causes spindle...
04 April 2013 2,463 20 View
