What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

Which are the specific cells instructed to constructing the "spikes" after a shot of a mRNA vaccine?

Hello, I am not a biology researcher, but I am working in intradisciplinary research and would like to know the answer to this question, which I have not being able to understand. This questions...

06 January 2021 3,845 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

What business models facilitate higher engagement of health tech start ups' user base so to retain them long term?

what business models are most popular among start-ups in general? There is very little research, if you can recommend some papers, that would be great!

17 March 2020 610 3 View

Can someone please provide references for the historical use of mycorrhizal fungi in agriculture?

Hello, I am interested in finding information regarding the historical use of mycorrhizal fungi in agriculture. I am having some difficulty finding this type of material. If you would be so kind...

21 November 2019 3,431 4 View

L cell WNT-3a-CM: why doesn't it work?

05 August 2019 1,264 5 View

Can I use RNA protect for RNA bacterial extraction inoculated on food matrix, and after that, dilute it?

Hi people! I would like to ask whether anyone of you have extracted RNA from bacteria on a food matrix. I have to inoculate bacteria on nuts, and after my processing tests, I need to extract RNA...

11 September 2018 7,343 2 View

What do I need to do to get you to send me the whole article named below?

by Trunnel and Holt: The Concept of Denial or Disavowal February 1974 Journal of the American Psychoanalytic Association 22(4):769-84

11 June 2018 252 3 View

What statistical analysis method should be used for analyzing the relationship between a nominal and ordinal variable?

The nominal variables are "working in a flextime schedule" and "working in a straight 8-hour schedule". The ordinal variables are scores from Likert-scales measuring Work-Family Balance and Family...

27 September 2017 1,877 11 View

If I want to do PCR of Cryptosporidium and Giardia to test, is it better to use a nested one?

05 June 2017 2,654 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View