I'm looking for a method to detect coenzyme Q10 in food (dairy products), using normal phase HPLC. Can anyone help me?
Thank you for your contribution.
Pamela
I mean working on shape and size without changing materials. The goas is to reduce joint and adhesion failures.
09 November 2023 5,013 3 View
If so, could someone please give some insights on the IPTG concentration, the length of the induction period, the temperature during induction, and the cell line that was used. The gene is cloned...
15 August 2023 831 1 View
Hello, I want to silence my gene of interest using ptdT-RNAi vector, so what I need to know is either if the amplicon must include the 5’UTR region, the ATG or both. I know that the length has to...
30 May 2023 307 1 View
I've seen some publications ask for one year before posting elsewhere. A university librarian informed me that no such posting is allowed, on any type of site, including for members only.
20 November 2022 9,144 10 View
I collected a seaweed sample from the Kenyan coast (Mkomani). I was looking for Caulerpa macroalgae. I am uncertain of the specific name of the pictured seaweed. Can you help me identify the species?
20 October 2021 7,530 7 View
Hello, I am doing a microsatellite PCR and the bands appear with a few definition, the electrophoresis conditions are the following, TBE buffer 0.5X pH 8.4 (according to Sambrook, Molecular...
29 August 2021 2,860 3 View
I am monitoring the degradation of contaminants using immobilized enzymes on carbon support. However, to complete the mass balance of the degraded reactant, I have to perform a mass balance on...
06 April 2021 6,802 2 View
I am struggling on how to calculate this. I know I need the MW, but I am not sure how these all tie together.
01 April 2021 4,795 1 View
Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...
08 February 2021 10,086 3 View
Hello, I am not a biology researcher, but I am working in intradisciplinary research and would like to know the answer to this question, which I have not being able to understand. This questions...
06 January 2021 4,195 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View