Hello everyone,

I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was completely yellow. Usually, when these bacterias are transformed they grow on MKL+ampicilin medium but present a purple colour and the medium is also red/purple.

I will be very grateful if someone knows why this could happen.

Thank you so much for your help.

Pamela B

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