If so, could someone please give some insights on the IPTG concentration, the length of the induction period, the temperature during induction, and the cell line that was used.
The gene is cloned in pET21b vector.
Cylia Salima Oulebsir
Hello there,
You can use E.coli BL21 (D3 strain) for your inductions (it's the one used basically everywhere).
In my experience, I always try 3 different Temperatures : 37°C, 25°C, and 18°C, and for the IPTG concentrations I also try 3 concentrations: 1mM, 0.5mM and 0.25mM.
The sample collection will be at 1H, 2H, 4H and overnight.
I hope you get your results!
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