Does anyone have an efficient protocol for human myocardium cells isolation?

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Why the PBS buffer precipitate?

How can i avoid precipitation in pbs? when i solve salts separately then add to a bowel there is no precipitation but when i want to solved them in a bowel after adding 3th salts the salts...

05 June 2018 5,999 4 View

Does Mesenchymal stem cell express Cardiomyocyte cells markers?

Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...

05 June 2018 6,629 4 View

Who can give me some information about DRPINs?

Are there companies that sell DARPins?Are there any websites that describe how to design DARPins?

09 October 2015 6,514 2 View

Has any one ever detected MCF-7 by flow cytometry with FITC-labeled antibody?

As you know FITC is a dim fluorochrome so I wanna know that FITC is a good fluorochrome for detection of MCF-7?Is there any problem to use of FITC for MCF-7 detection by flow? actually we treated...

31 December 2014 1,537 12 View

Is Isotype control antibodies (mouse IgG) for flow cytometry general?

I'm working on CD34-FITC and used CD34- isotype control antibodies from miltenybiotec company, but the CD34-FITC control is not available in our lab right now but I have CD86-FITC isotype control...

10 November 2014 6,836 8 View

How can I analyze rare cells by fluorescence microscope, not by FACS?

I want to detect some rare cells and I can not do analysis by FACS because of rarity and I have to detect them by fluorescence microscope but I need a protocol for this assay.

10 November 2014 3,554 13 View

Who can send me the photo of normal hematopoitic stem cells which isolated from cord blood?

I wanna know the morphology of them

02 March 2014 9,448 1 View

Is there any site for comparing the levels of expression specific marker between two cells?

I have two cells and i want to know which of them express specific marker more ?

11 December 2013 9,090 3 View

Which company produces specific antigen?

I want to order some antigen and want to know which company is best?

10 November 2013 8,446 4 View

Does anyone know which cancer cell lines (Cancer stem cell, tumor or metastatic) are positive in EpCAM?

I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.

08 September 2013 8,103 2 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View