Why the PBS buffer precipitate?

How can i avoid precipitation in pbs? when i solve salts separately then add to a bowel there is no precipitation but when i want to solved them in a bowel after adding 3th salts the salts...

05 June 2018 5,858 4 View

Does Mesenchymal stem cell express Cardiomyocyte cells markers?

Real-Time is done and Mesenchymal stem cells had expression of cTNT, CTNN1 and other Cardiomyocyte cells markers is it possible or cTNT is an specific Cardiomyocyte marker and something wrong is...

05 June 2018 6,515 4 View

Who can give me some information about DRPINs?

09 October 2015 6,419 2 View

Has any one ever detected MCF-7 by flow cytometry with FITC-labeled antibody?

As you know FITC is a dim fluorochrome so I wanna know that FITC is a good fluorochrome for detection of MCF-7?Is there any problem to use of FITC for MCF-7 detection by flow? actually we treated...

31 December 2014 1,428 12 View

Is Isotype control antibodies (mouse IgG) for flow cytometry general?

I'm working on CD34-FITC and used CD34- isotype control antibodies from miltenybiotec company, but the CD34-FITC control is not available in our lab right now but I have CD86-FITC isotype control...

10 November 2014 6,729 8 View

How can I analyze rare cells by fluorescence microscope, not by FACS?

I want to detect some rare cells and I can not do analysis by FACS because of rarity and I have to detect them by fluorescence microscope but I need a protocol for this assay.

10 November 2014 3,439 13 View

Who can send me the photo of normal hematopoitic stem cells which isolated from cord blood?

I wanna know the morphology of them

02 March 2014 9,358 1 View

Is there any site for comparing the levels of expression specific marker between two cells?

I have two cells and i want to know which of them express specific marker more ?

11 December 2013 8,991 3 View

Which company produces specific antigen?

I want to order some antigen and want to know which company is best?

10 November 2013 8,346 4 View

Does anyone know which cancer cell lines (Cancer stem cell, tumor or metastatic) are positive in EpCAM?

I wanna set up a control for my test I want to use some cell lines that the cells are positive in EpCAM.

08 September 2013 7,994 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View