i am trying to apply Buiret method for determining the protein content using spectrometer. it is a very accurate method but very sensitive, presence of carbohydrate and starch especially interfere with the measuring and makes partial obscurity. if we get rid of starch content in the sample preparation or succeed in precipitating it in the final measuring tube the measuring will be satisfying.
How about precipitating proteins using a solvent like acetone, separate it, redissolve and apply Buiret method
Hi Sameh, an alternative approach can be to digest the starch and other polysaccharides with a small amount of enzyme (amylase) and seperate the small carbohydrate fragments from the sample by ultrafiltration.
Both answers got pro's and cons.
1. Precipitation, as well as re dissolution may be non quantitative;
2. Amylase is a protein, of course. Very careful standardization is a must!
3. What I would do firstly? Go to google scholar, carefully select search-items and see, how researchers' generations dealt with this problem.
Plz let me know how to remove carbohydrates from the defeated millet sample
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