We need to determine the cell volume of cardiac myocytes and have frozen sections from E12.5 E15 and E18.5 mouse fetal hearts for staining. WGA seems to be too blurry, and H&E doesn't give definition of the cell surface to outline the cell boundary. As there are other analyses which use these frozen sections, we need to use alternating sections for the different stains. Is there a good stain/technique that can be applied to frozen 5 micron sections of fetal mouse hearts.