Refer to uploaded video. When using serial EM in low dose mode the beam is moving when switching between focus and record. This results in bad images that are blurry. Anybody encounter this before? Anyone know how the fix this?
Jason Jones It appears that you have two inquiries.
First, while utilizing serial EM in low dosage mode and alternating between focus and record, why does the beam move? Low dose mode allows focusing and tracking operations in a separate location, reducing beam exposure.However, this requires the beam to move between different areas, controlled by image shift coils that deflect the beam using a magnetic field. The amount of image shift depends on magnification, spot size, and beam intensity.
Second, how to fix the blurry images caused by the beam movement? One method is to modify the exposure duration or the camera binning to change the beam strength, while maintaining the same magnification and spot size for the focus and record regions.
Thank you Alvena Shahid for you answer. I added a few seconds to the settling time for record mode in serial EM and this helped with obtaining clear images.
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