Bone marrow mesenchymal stem cells (BMMSCs) are the origin of many tissues such as fat, bone, and cartilage. Moreover, it can contribute to the regeneration of damaged tissue. However, no evidence has shown that mesenchymal stem cells may have any tumor formation or other serious adverse effects. In this study, mesenchymal stem cells (mesenchymal) cells were separated from the bone marrow of mice at different ages (3, 6, and 9 weeks), cultured in a DMEM environment, and their characteristics were characterized using surface markers associated with mesenchymal stem cells (mesenchymal stem cells) via flow cytometry, in addition to... To evaluate the rate of cell proliferation at different periods of cell culture (3, 9, and 15 days), with the aim of evaluating the effect of age on the number of isolated cells and their various characteristics. Our results showed that the number of cells increases at young ages compared to older ages of the mouse’s life. In addition to that The number of cells was similar in mice aged 6 weeks and 9 weeks on the 51st day of culture in the laboratory, in addition to the presence of variation in cell shapes during the days of culture in the initial passage from the third day until the fifteenth day at the specified age. In addition to the strong expression of MSC markers. Moreover, BMMSCs derived from 3-week-old mice had a higher proliferation rate than those derived from older mice, while the cell proliferation rate was similar in 6-week-old and 9-week-old mice on day 15 of cell culture. In conclusion, BMMSCs maintained their MSC characteristics in all study groups and their numbers increased in the early stage of life compared to later stages of life which may be due to aging of mice. The direct relationship between age and cell number in mice still needs more evidence. However, it remains important to characterize the genetic properties of mesenchymal stem cells via expression patterns

Genetics at different stages of the life of mice.