Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have access to sea water). Is just for small/medium scale culture. Thank you very much.
If you have access to seawater, I would suggest sterilizing by micro filtration (0,22 microns) and then add the well known F/2 medium. It is a complete nutrient medium used at a dose of 1 ml/l and it works fine with Macroalgae.
Jose Pedro Canavate Thank you! I am new in this reaserch topic, so... too much to learn. Thanks again and kind regards.
Have you tried adding 35 grams of salt to a beaker, and then add tap water until the total mass weighs 1,000 grams.
You have to stir/mix until the salt is completely dissolved in the water.
Tap water often contains lots of natural minerals found in seawater, such as magnesium and calcium
The above method is applicable when you want an expensive medium. Note that you may have problem trying to replicate the medium for experiments.
If you want to carryout out reproducible experiments, you will need to use a standard medium like F/2.
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