I am trying to find a recipe to add 2 deoxyglucose food for Drosophila. In Munica dus et al., 2011 paper, they have mentioned feeding flies with 400mM 2deoxyglucose but I wonder how you make it?Is it just on a plug and feeding them on that plug?or adding that to food?
According to the paper you mention, they appear to add glucose directly to the water and agar solution before allowing it to cool and harden. You can also find similar agar/glucose recipes with fruit juices in the attached article.
Another recipe to consult:
Yeast-glucose medium for Drosophila (Cold Spring Harbor Protocols)
30g agar, 100g dry yeast, 100g glucose, 15ml Nipagin M (10% [w/v] in 95% ethanol) (Nipa Laboratories Ltd.)
Mix the three dry ingredients in a blender and add tap water to 1 liter. Bring to a boil slowly and simmer to thicken. Then add the Nipagin M (fungal inhibitor).
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