Is it possible to extract intact immunoglobulins from fresh frozen immunoglobuline-producing tumor biopsies?

For example using Ripa buffer? Or will the immunoglobulins fall apart?

10 November 2015 4,212 1 View

Can I use western blot to assess immunoglobulin expression in cell lines?

I tried staining cytospins of the cell lines for immunoglobulins but frequently there is quite a lot of background. Could I use western blotting?

07 August 2015 2,901 4 View

I cannot detect Epstein-Barr virus-encoded RNA (EBER) in the Farage cell line?

Farage is described as Epstein-Barr virus-positive, however I cannot detect expression of EBER by in situ hybridization. Can anyone explain this? http://www.atcc.org/products/all/CRL-2630.aspx

10 November 2014 7,374 5 View

How long does the effect of lytic cycle-inducing agents (TPA, sodium butyrate, IgG) last on Epstein-Barr virus (EBV)-producing cell lines?

10 November 2014 6,877 8 View

Can the Epstein-Barr virus (EBV) be episomally present in B-cells without associated expression of viral RNA or protein?

09 October 2014 9,608 1 View

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08 September 2014 7,474 4 View

Does anyone have experience with culturing OCILY3 and OCILY10 cells?

Hi everyone.I've been culturing OCILY3 and OCILY10 cells in RPMI1640 with 20% FBS, 10% FBS or in advance DMEM with 10% FBS (in all cases penicillin/streptomycin and glutamax were added) but in...

06 July 2014 5,327 7 View

What is known about metastasis of B-cell lymphoma?

06 July 2014 7,208 8 View

What is the best reference/housekeeping gene for PCR on lymphomas?

I was wondering if anyone working with lymphoma could tell me which reference/housekeeping genes they include in their PCR experiments?

04 May 2014 405 4 View

Is 30-60% methanol (like Thinprep PreservCyt) a safe fixative for EBV-infected cells?

Does this methanol concentration kill the EBV particles? Or what would be a better fixative?

07 August 2013 887 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View