Garry Nolan's website probably has all the info you're looking for:

http://web.stanford.edu/group/nolan/tutorials/retpkg_7_phx_sys.html

Garry Nolan's website is indeed the best for background info.

We used transduction of viral oncogenes into keratinocytes and published the obtained results. It worked fine for us, see M&M. You may want to have a look at the attached publication.

good luck!

Article Specific betapapillomaviruses associated with squamous cell ...

Yeah, I did faced low transfection efficiency of phoenix cells.

I am also facing the same problem. If you have optimized the protocol please share it.

 Dear all,

I am confused, are you talking about transfection or transduction. The first is generally considered non-viral and the second is using a virus.

So if the issue is to transduce your cells with a retrovirus, I can recommend two options for enhancing the infectivity

- one is to use a a chemical method similar to polybrene without the toxicity and drawbacks issues. The reagent is called Lentiblast, its patent-protected chemical composition allows simultaneously neutralizing electrostatic repulsion between membrane and viral particles and enhancing viral fusion with cell membrane.

http://www.ozbiosciences.com/transduction-infection/75-lentiblast-transduction-infection-lentivirus-booster.html

- second is to use a magnetic  nanoparticles based methods called Magnetofection that showed a large success in a variety of models

http://www.ozbiosciences.com/lentivirus-retrovirus/68-viromag-rl-magnetofection-reagent-viral-transduction.html

Now if you are intending to transfect your cells with plasmid DNA in order to produce retrovirus, Magnetofection or CaPO kit are good options.

if you need more info please do not hesitate to contact us at; [email protected]

Good Luck

Olivier