What is the maximum length of DNA that can be packaged into retroviral vector?
Do you guys know how to precisely analyze the length of glass fibers by ImageJ? I am trying to analyze the glass fibers image that was taken by microscopy to get the length of fibers and their...
25 July 2023 9,208 0 View
I have transduced mammalian cells with lentivirus to express an exogenous gene. The transduced cells were sorted out on a FACS machine based on the immunostaining of the protein encoded by the...
02 June 2021 3,694 6 View
I extracted genomic DNA and total RNA from fruiting bodies and mycelium of basidiomycota in a daily routine. Some pigments can not be remove from DNA/RNA with column. Consequently, DNA/RNA...
30 April 2014 4,054 4 View
I am studying enzymatic transglyosylation of activated glycosyl donors. I submitted several papers, however, I don't really know who pointed out that nitrophenyl glycoside is one of the suitable...
05 November 2012 7,737 2 View
To improve organic surface for the application of hybrid materials is required the molecular bridge( as interaction) .Kusano Sensei knows my work due to the special support for our special book...
01 January 1970 1,550 2 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
What are the reasons why we need to practice proper post-harvest handling including packaging, after harvesting fruits and vegetables?
02 July 2024 9,999 2 View
How is active packaging as a method of preserving fruits and importance of handling packing of fruits and vegetables in prolonging their life and maintaining their quality?
02 July 2024 2,796 2 View
I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!
21 June 2024 3,484 1 View
How can contexual bandits (used in Recommendation Systems) be implemented in code via library package ?
18 June 2024 3,196 6 View