Devi Thiagarajan

4 Questions 6 Answers 0 Followers

Questions related from Devi Thiagarajan

Can gel fitration column be used for seperating two proteins of 10kd difference?

I need to do gel filtration with a complex and third protein. Thinking of doing FPLC combined gel filtration column. The complex is made of two proteins of 50KD and 15 kD. Third protein is 36kd. I...

25 November 2014 5,836 8 View

Can anyone help with protein loss while purification?

I am trying to purify two proteins. Protein 1, is around 16kd, sequence verified, expression verified via western blotting. problem is induction. it is not induced. i tried various concentrations...

17 October 2014 5,968 9 View

Can anyone help me with bacterial protein expression cloning?

I am trying to subclone gene of my interest along with tag from pcdna3.1 to pET28b vector. As the insert has its own ATG, gene of interest and fusion tag in frame, I want to cut the whole lot and...

11 September 2014 6,151 5 View

Does anyone have a protocol for an efficient retroviral transduction using phoenix cells?

I need to know more about phoenix cells.

20 May 2014 4,136 5 View