Which dose do you expose RAG1 deficient mice for lethal-irradiation?

Hello, I'd like to apologize for my English in advance, I'm still trying to polish it. We are performing several bone marrow transplantations (BMT) or fetal liver transplantations (FTL) to...

05 November 2020 4,239 1 View

What is the Method of Moment theory and what differentiates it from the MLFMM solver?

Hi everyone, It's been quite a time since I have started searching the web for clear explanations on the Method of Moment (MoM) theory for electromagnetic field computing. Does anyone know a...

23 September 2020 2,763 4 View

Innovation, Ideas permeability. Which factors make an idea to be commercialized?

There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...

13 July 2020 7,807 12 View

Are there someone thinking about a simulation software for evacuation/circulation tasks including COVID-19 propagation?

I think it would be interesting to implement the propagation of the COVID-19 virus in some simulation software for the circulation of people, for a population diverse in ages, heights, etc.,...

30 June 2020 7,481 14 View

COVID-19, Lessons learned ?

It should be mandatory to learn from what life teaches us. With COVID-19, we have been forced to buy sanitary materials because in our country there was no industry to do so (dependency) and we...

30 June 2020 3,018 36 View

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04 March 2020 2,681 3 View

Some good "R" package for Raman data analysis ?

I need some software for RAMAN data analysis, I prefer some good R package

08 April 2018 1,104 1 View

What importance does the study of theory of mind in patients have with the psychiatric diseases?

The ability to empathize and understand the perspective of the other seems to be altered in diseases such as the schizophrenia.

17 September 2014 6,456 6 View

Why nikel ion does not bind to Ni-NTA column after APS treatment?

I treated Ni-NTA column with 10% APS because I purified a protein using a buffer containing DTT and it tourned brown. After APS treatment I wash the column with water and then I stripped it with...

10 July 2014 3,577 7 View

Literature Update tool!

What tools do you use to stay up to date with the last publications in your filed?, google scholar alert.? How to avoid read over and over again that long lists in different papers that you...

10 November 2013 6,450 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View