RTD Step analysis with matlab?

Hello to all my dear colleagues. I need to get my E(T) curve for my reactor. I already have the measures but I ignore how to procede with the data analysis. I have got Matlab but i am not sure if...

21 June 2020 2,675 5 View

Help about chemical sludge characterization?

Hello. I did run some coagulation / flocculation experiments[FS1] and I have been requested to characterize the sludge. I am ignorant on how to so. I was only requested to do SEM, XRD and Zata...

16 June 2020 8,715 7 View

In solar heterogeneous photocatalysis, is solar geometry relevant?

My project is supposed to considered solar irradiation as an important variable for heterogeneous photocatalysis. I haven't found any study that considers solar geometry as a variable of study in...

30 January 2020 744 3 View

Why do I have a Jagged line on my qPCR amplification?

I am performing a qPCR standardization of some MGB probes. I have tried just a few times but I don't want to waste reagents, every time I tried the assay, there is a jagging line. I am thinking...

13 November 2019 4,554 3 View

Do you have information about monoglycerides determination by Normal phase HPLC using a ELS detector?

We determine monoglycerides by normal phase HPLC using a ELS detector obtaining reliable results. Now we have a new equipment (SHIMADZU UFLC) with the same type of detector. However, when...

09 October 2019 1,244 3 View

Can a probe work with less Tm than the primers?

I found 2 interesting articles for the detection of bacteria at Phylum level with MGB probes, claiming that they work. I want to use that primers however when I use the oligoanalyzer tool, it...

08 July 2019 6,409 3 View

Which are the best methods to dye plastic microfibers in a sample?

We are trying to identify microfibers in water samples

28 April 2019 2,162 3 View

Protocol for polymer stainning with Ruthenium tetroxiode?

I cannot find a comprehensive protocol for stainning polymers with Ruthenium tetroxide solution. I would like to know the instructions and safety precautions for stainning and handling of...

04 November 2018 7,875 2 View

Does anyone has a protocol for measuring dopamine using and ELISA? . Does ELISA id DA levels or only TH or DAT?

Hi All, I'm completely new in the dopamine world and ELISA. I collected fresh brain samples and I have them in -80C. Do you have any protocol for ELISA brain sample preparation? I know the kits...

07 August 2018 6,428 3 View

Measurement soil pH in situ?

Somebody know how measurement the soil pH, according of mexican normative in laboratory I can use CaCl2 and I need wait 30 min, but I am thinking to determine soil pH in field with way faster that...

05 June 2018 8,337 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View