How to get total dna solubilization?

Carlos Vázquez Pérez @Carlos-Vazquez-Perez-3

22 December 2023 1 109 Report

I have a question, I have noticed that when I extract dna, in the last step when I resuspend in either 50 microliters of TE buffer or water, the total precipitated dna doesn`t solubilizes. I haved tried to incubate for one hour at 65º degrees but it doesn`t always work, does someone have some tip?

Carlos Vázquez Pérez

I want to add, I am working on extracting T. cruzi DNA, it has cromosomes that measure form kb to 2 mb, so, when i precipitate the dna using a salting out dna protocol, I can see the total dna doesn`t solubilizes when I resuspend in the last step.

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