We are designing the test where we will select a specific bacteria from different samples and we will seek to find this bacteria with the characteristics that we are looking for (genes), it will be evaluated by biosafety tests, sequencing, etc. (If it is safe and has the gene, it is classified as apt, otherwise it is discarded ).

We have several variation factors such as the type of samples (food, environmental, feces), culture conditions (aerobic & anerobic) and media (2 different). The number of isolates will be equally distributed according to the combination of each factor.

I have searched the literature and have not found a statistical test for qualitative variables that can help me to determine the sample size.

The closest method that I have found is where I should search for similar studies to determine the probability of finding the desired characteristic and from there estimate the sample size. Although I'm not sure if it's the best method.

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