Where to get icons for a research paper?

Hi, I'm trying to create nice illustrations for my paper. Are there good resources free for educational non-profit purposes you recommend? Moshe

24 March 2020 994 3 View

How to mention a missed relevant research?

Hi, After the paper was written (and submitted) I discovered a previous relevant research having much in common with my paper. I will have a chance to edit the paper before publishing it. What...

27 August 2019 6,601 4 View

Can i dilute protein for western blot with water instead of the Lysis buffer?

After preparing the cell lysate and after protein quantification, if i don't have any lysis buffer left, can I use water (+protease inhibitors) to prepare the protein sample for loading with the...

28 January 2019 5,159 7 View

How should I change the voltage and run time in gel electrophoresis according to the size of the gel?

I apologize if this is a basic question. I had a hard time finding answers to it. I try to deepen my understanding of gel electrophoresis and wonder how should I change the voltage and run time...

09 December 2018 5,702 4 View

What protocol can i use for epithelial cells intracellular cytokines staining?

I want to know what is the purity of the isolated epithelial cells samples.

07 November 2016 6,118 2 View

Viral conjunctivitis research collaboration?

Seeking collaborator for in-vivo testing of a repurposed compound to treat viral conjunctivitis. It works in humans but seeking animal model to verify in.

10 January 2016 2,959 2 View

What are other applications of spin-tensors in physics?

Spin-tensors are used in advanced general relativity and to a lesser extent in formulations of quantum field theory based on the Dirac equation. Are there any other known applications of...

23 February 2015 8,173 1 View

Some RNA viruses are polyadenylated , evolution should had led them to avoid A tailing?

Dominique. If polyadenylation has a role in RNA degradation , would you expect RNA genomes to carry such a signal,? Viruses apparently would do everything to stabilize their large genomes , yet...

01 January 1970 8,362 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View