6 Questions 178 Answers 0 Followers
Questions related from Gary S. Laco
Hello, I am looking for options on how to handle the following issue. I am aligning hundreds of amino acid (AA) sequences for HIV-1 Gag. I am interested in the protease cleavage sites in Gag and...
07 August 2025 9,884 3 View
I have LC/MS output RAW files (Excel file containing multiple Sheets, with each Sheet containing the peak data for one sample) and I want to quantitate those peaks using a lipid Target list. I...
09 March 2021 3,776 2 View
when using E. coli BL21 pLys, grown in Terrific Broth, for toxic/non-toxic protein expression I normally induce protein expression at ~0.5 OD using IPTG, and the resulting induced cultures double...
25 February 2016 1,938 14 View
I have used the megaprimer PCR approach in the past to make mutations with no problems, however, the mutagenic primers typically mutated only one or two codons and now I want to mutate at least 25...
10 March 2014 9,655 4 View
I want to clone a short DNA fragment of ~80 bp. The DNA fragment will be made by annealing two oligos together that were designed to generate 5' and 3' overhangs for the RE site, so the DNA...
28 February 2014 477 6 View
I am looking for a software program that can analyze a protein coding region of DNA and find where restriction sites can be added without changing the coding sequence. I did find the program...
28 February 2014 5,906 16 View