Gary S. Laco

5 Questions 149 Answers 0 Followers

Questions related from Gary S. Laco

Software to quantitate known lipid peaks from LC/MS output RAW files?

I have LC/MS output RAW files (Excel file containing multiple Sheets, with each Sheet containing the peak data for one sample) and I want to quantitate those peaks using a lipid Target list. I...

09 March 2021 3,719 2 View

Terrific Broth and protein expression in E. coli, induction OD?

when using E. coli BL21 pLys, grown in Terrific Broth, for toxic/non-toxic protein expression I normally induce protein expression at ~0.5 OD using IPTG, and the resulting induced cultures double...

25 February 2016 1,881 14 View

Megaprimer PCR that mutates 75 bases or more?

I have used the megaprimer PCR approach in the past to make mutations with no problems, however, the mutagenic primers typically mutated only one or two codons and now I want to mutate at least 25...

10 March 2014 9,606 4 View

Has anyone ever cloned short DNA fragments under 100 bp into a plasmid?

I want to clone a short DNA fragment of ~80 bp. The DNA fragment will be made by annealing two oligos together that were designed to generate 5' and 3' overhangs for the RE site, so the DNA...

28 February 2014 429 6 View

Is there a software program to add restriction sites to protein coding sequences without changing protein coding sequence?

I am looking for a software program that can analyze a protein coding region of DNA and find where restriction sites can be added without changing the coding sequence. I did find the program...

28 February 2014 5,846 16 View